stationary phase, in analytical chemistry, the phase over which the mobile phase passes in the technique of chromatography. ... Typically, the stationary phase is a porous solid (e.g., glass, silica, or alumina) that is packed into a glass or metal tube or that constitutes the walls of an open-tube capillary.
What happens in the stationary phase in chromatography?
This is called chromatographic development. In all chromatography there is a mobile phase and a stationary phase. The stationary phase is the phase that doesn't move and the mobile phase is the phase that does move. The mobile phase moves through the stationary phase picking up the compounds to be tested.
What is the purpose of the stationary phase?
The stationary phase remains fixed in place while the mobile phase carries the components of the mixture through the medium being used. The stationary phase acts as a constraint on many of the components in a mixture, slowing them down to move slower than the mobile phase.Aug 15, 2020
What is the stationary phase in HPLC?
There are two phases for HPLC: the mobile phase and the stationary phase. The mobile phase is the liquid that dissolves the target compound. The stationary phase is the part of a column that interacts with the target compound.
In which chromatography stationary phase is liquid?
Technique Stationary phase Mobile phase
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*Paper chromatography solid (cellulose) liquid
*Thin layer chromatography (TLC) solid (silica or alumina) liquid
*Liquid column chromatography solid (silica or alumina) liquid
Size exclusion chromatography solid (microporous beads of silica) liquid
Is HPLC is liquid-liquid chromatography?
High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. ... Chromatography can be described as a mass transfer process involving adsorption.
Is the stationary phase solvent?
Term Definition
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Mobile phase or carrier solvent moving through the column
Stationary phase or adsorbent substance that stays fixed inside the column
What is the stationary phase in reversed-phase HPLC?
The term reversed-phase describes the chromatography mode that is just the opposite of normal phase, namely the use of a polar mobile phase and a non-polar [hydrophobic] stationary phase.
What stationary phase is used in reverse phase chromatography?
hydrophobic stationary phase
What are the most common mobile phases in reversed-phase HPLC?
Similarly, mobile phase A is the weaker, aqueous mobile phase. Historically, the three most common reversed-phase LC organic solvent choices are acetonitrile, methanol, and tetrahydrofuran.Sep 30, 2018
What is stationary phase in liquid liquid chromatography?
The stationary phase in column chromatography is most typically a fine adsorbent solid; a solid that is able hold onto gas or liquid particles on its outer surface. ... Then the adsorbent solid (usually silica) is tightly packed into the glass tube to make the separating column.Aug 15, 2020