Qubit Fluorometers detects fluorescent dyes that are specific to the target of interest. With a Qubit Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Invitrogen Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample.
What is Qubit quantification?
The Qubit fluorometer is a lab instrument developed and distributed by Invitrogen (now part of Thermo Fisher) that, among other applications, is used for the quantification of DNA, RNA, and protein.
What is the difference between Qubit and NanoDrop?
Devices using DNA binding dyes such as the qubit measure DNA concentrations directly, whereas the nanodrop quantifies anything that fluoresces at the approximate wavelength of DNA.
Is Qubit a spectrophotometer?
Qubit platforms give sensitive, accurate and precise measurements compared to UV absorbance instruments. The same concentrations of DNA were measured in 10 replicates using a microvolume spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates.
Can Qubit measure RNA?
New—The Qubit™ 4 Fluorometer can now measure RNA quality in addition to providing accurate quantitation of dsDNA, ssDNA, RNA & protein.
How does Qubit measure DNA concentration?
The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA.Mar 3, 2016
How do you quantify DNA using Qubit?
https://www.youtube.com/watch?v=RRKZN--7jqg
Does Qubit measure degraded RNA?
The Qubit RNA IQ provides a fast, simple method to check whether a RNA sample has degraded (Figure 4). A gel or microfluidic-based electrophoresis is still recommended to obtain detailed fragment size information or size distribution of the RNA sample.
How does Qubit RNA work?
The Qubit fluorometer uses fluorescent dyes that emit signals only when bound to the specific target molecules (DNA or RNA) even in the presence of free nucleotides, degraded nucleic acids, or protein contaminants.
Which is better NanoDrop or Qubit?
The sensitivity of the Qubit can be as low as 10 pg/mL, which is far superior to the Nanodrop. So, if you worry your samples have very low concentrations, it is best to use the Qubit. Thus, if you have samples with DNA and RNA in, it is best to use the Qubit to determine the concentration.
What is the difference between NanoDrop and spectrophotometer?
Another useful feature of the NanoDrop is the ability to measure CyDye incorporation into microarray probes. Using the NanoDrop to check probe quality is now a standard procedure in our lab. Unlike other small spectrophotometers, the NanoDrop is able to scan samples throughout the 220-750nm range.