anyway , DMEM is basically MEM with additional amino acids , vitamins and proteins to prevent the adhesion of the cells to the sides of the flask . DMEM is richer in a variety of nutrients than MEM and is a very good choice for many cell types. Cells will grow faster and deplete the medium slower in DMEM.
What is in EMEM media?
Eagle's minimum essential medium modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.
What is MEM in cell culture?
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells.
What is MEM media used for?
MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Earle's salts has broadened the usefulness of this medium.
What is MEM in animal cell culture?
Minimal Essential Medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in Science that can be used to maintain cells in tissue culture. This permits the cultures to be kept for somewhat longer periods without refeeding".
Whats the difference between RPMI and DMEM?
RPMI is a media used widely to culture mammalian cells in suspension culture. DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.
What is in Dulbecco's modified Eagle medium?
Dulbecco's Modified Eagle's Medium (DMEM) is modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. Contains 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.
What is MEM made of?
It is based on 6 salts and glucose described in Earle's salts in 1934: (calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, sodium phosphate and sodium bicarbonate), supplemented with 13 essential amino acids, and 8 vitamins: thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3