Microplate Readers, or Plate Readers, are a type of spectrophotometer. Both use either a monochromator system or filter-based optics and these lab instruments both collect useful measurements across a variety of methods.16 Aug 2021
How does fluorescence plate reader work?
The emission system of the plate reader uses polarizing filters to analyze the polarity of the emitted light. A low level of polarization indicates that small fluorescent molecules move freely in the sample. A high level of polarization indicates that fluorescent is attached to a larger molecular complex.
What does an ELISA plate reader do?
An ELISA reader measures and quantitates the color differences in the 12 wells of the plate. ELISA readers or micro plate readers do spectrophotometry; they emit light at one wave length, and measure the amount of light absorbed and reflected by an object such as a protein.
How much is a plate reader?
A $4,000, filter-based reader with a halogen lamp can read the vast majority of enzyme-linked immunosorbent assays (ELISAs), for example, and a $12,000 UV-vis absorbance reader outfitted with a tunable monochrometer is just fine for most colorimetric assays, including DNA and protein quantitation.13 Oct 2016
A microplate reader is a laboratory instrument that is used to measure chemical, biological or physical reactions, properties and analytes within the well of a microplate. A microplate consists of small wells in which separated reactions take place.