Press the push button to the first stop (this corresponds to the set volume of liquid). 2. Hold the pipette vertically and immerse the tip in the liquid (refer to Guidelines for Good Pipetting on page 9). Release the push button slowly and smoothly (to top position) to aspirate the set volume of liquid.
How do you take apart a pipette?
https://www.youtube.com/watch?v=21CO53UUkV8
How do you calibrate a pipette step by step?
https://www.youtube.com/watch?v=fyOV9iyZMNY
Can you calibrate a pipette?
To check the calibration of a pipette, you will need the pipette, pipette tips, distilled water, a beaker, a thermometer, a balance, and weigh boats. The balance needs to be specific to micrograms to calibrate micropipettes with a maximum of 1 µL. You won't need more than 5 mLs of water.
How do you know if a pipette is accurate?
The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh 0.001 g. In other words, if your pipette is accurate, the amount of water you dispense will equal the amount the water weighs.21 Jul 2014
How do you calibrate a 25 mL pipette?
https://www.youtube.com/watch?v=0NmbYqERfoo
What is the first thing you should do when using a pipette?
- Select the volume.
- Set the tip.
- Press and hold the plunger at the first stop.
- Place the tip in the liquid.
- Slowly release the plunger.
- Pause for a second and then move the tip.
- Insert the tip into the delivery vessel.
- Press the plunger to the second stop.
How do you use a pipette step by step?
https://www.youtube.com/watch?v=QGX490kuKjg
How does a Gilson pipette work?
Air pressure increases inside the shaft and the tip. The compressed air pushes the liquid out of the tip. The push button is pressed again. The piston moves down and expels the liquid out of the capillary.